Karyotyping is often used to detect chromosomal anomalies of the fetus. This fetal cells for signs of numerical (eg. As trisomy) and structural (eg. As deletion) chromosomes are anomalies investigated that correlate with enzyme defects, growth retardation or one of the more than 75 other known syndromes.
For this purpose (puncture of the amniotic sac) or chorionic villus sampling (tissue sample) are obtained by means of suitable cells amniocentesis, which ideally should not possible be contaminated with maternal cells to allow a clear unambiguous diagnosis.
In the cultivation of these fetal cells, it is important that you as soon as possible to many clones grow no cultural-artifacts (induced aberrations) have what could otherwise cause erroneous results.
You should also be of the type fibroblasts and epithelial cells no easier for subsequent processing as possible. Only in very exceptional cases, where there any reason whatever comes at no fibroblast growth, it makes sense that epithelial cells grow but time-shifted backward by the sample is not completely lose.
Amniocentesis is performed usually 14 to 20 weeks gestation, a biopsy may be already in the 9 ½ -12. Week of pregnancy can be performed.
For optimal growth of amniocytes and chorionic villi cells, the use of pre-tested complete media makes sense with properties that correspond to the previous claims. This applies to Amniogrow Plus and Amnioquick Medium.
It promotes rapid growth of fibroblasts and is therefore equivalent to the aforementioned characteristics very well. The amniotic fluid cell media range is regularly reviewed by independent specialist laboratories for cytogenetics in the routine. The special closeness to clinical diagnosis has created over the years of quality and special safety.